摘要
目的:调查产超广谱β-内酰胺酶铜绿假单胞菌在医院烧伤病房中的流行情况及脉冲场电泳特征。方法:采用脉冲肠凝胶电泳技术对铜绿假单胞菌进行测定,并用双纸片扩散法筛选产超广谱β-内酰胺酶铜绿假单胞菌。结果:30株铜绿假单胞菌共筛选出7株(占23.3%)产超广谱β-内酰胺酶菌株;通过脉冲场指纹图谱并显示7株产超广谱β-内酰胺酶菌株都是同一基因型;其中3株来自烧伤科物体表面;4株来自烧伤病人创面;其对头孢他啶、头孢哌酮、头孢曲松、头孢噻肟、头孢吡肟、哌拉西林、亚胺培南、氨曲南、庆大霉素、妥布霉素、阿米卡星、环丙沙星和氧氟沙星等13种抗菌药物呈现全耐药现象。结论:在医院感染产超广谱β-内酰胺酶铜绿假单胞菌的分子流行病学研究中,脉冲场凝胶电泳是一种较好的研究方法。
Objective： To investigate the prevalence of extended - spectrum β- lactamases （ ESBLs ） producing Pseuclomonas aeruginosa in burn wards. Mcthods：P. aeruginosa was estimated with the pulsed -field gel electrophoresis （PFGE） and and Kirby - Bauey bacterial susceptibility test was used to screen for strains producing ESBLs. Results： Seven in 30 P. aeruginosa strains （23.3%） ,3 strains from object surface and 4 strains from patients＇ wounds were identified to be ESBLs producing which belonged to the same PFGE genotype D and were completely resistant to all the following 13 antibiotics： gentamicin, amikacin, ciprofloxacin, kfloxacin, cefotaxime, ceflriaxone, tobramycin, aztreonam, cefoperazone, cefepime, piperacillin, ceftazidime and imipenem. Conclusion：PFGE is an effective method to study the molecular epidemiology of ESBLs producing P. aeruginosa in hospitals.
出处
《中国卫生检验杂志》
CAS
2006年第2期143-144,共2页
Chinese Journal of Health Laboratory Technology
