摘要
目的:比较骨形态发生蛋白2在犬自体、异体、冷冻异体气管移植体内诱导软骨再生的作用。方法:实验于2004-03/10在第四军医大学唐都医院实验外科完成。21只杂种犬随机等分为3组,自体移植组、异体移植组、冷冻后的异体移植组。各组动物颈部切除4环气管段作为移植体,植入骨形态发生蛋白2后埋入腹腔大网膜中。骨形态发生蛋白2均以胶原溶液作为缓释载体,用注射器直接注入各软骨环间。术后5周处死实验动物,通过对标本组织学观察、碱性磷酸酶活性和钙含量测定,对各标本的新生软骨进行观察和定量分析。结果:各组实验犬均存活到预杀期。①定量组织学观察:各实验组骨形态发生蛋白2植入区均可见新生软骨细胞和软骨岛,HPLAS-1000病理图像分析系统计算各环间新生软骨的像素面积,自体移植组、异体移植组、冷冻后的异体移植组的新生软骨面积差异有显著性犤2573.6±738.4,1691.3±743.6,2482.9±827.5,F=5.711,P<0.05犦自体移植组和深低,而温冷冻后的同种异体移植组差异无显著性。②碱性磷酸酶活性和钙含量测定:自体移植组、冷冻后的异体移植组与异体移植组均差异有显著性犤体移植组:(7.00±0.50)μkat/g,(5.48±1.15)mg/g;冷冻后的异体移植自组:(7.00±0.83)μkat/g,(5.03±0.91)mg/g;异体移植组:(5.00±0.67)μkat/g,(3.57±0.98)mg/g,P<0.05犦,自体移植组与深低温冷冻后的同种异体移植组间无显著性差异(P>0.05)。结论:骨形态发生蛋白2能在气管移植体内有效地诱导出新生软骨,在冷冻后的异体气管移植体内的作用效果与自体气管移植体内相似,明显优于异体气管移植体。
AIM： To compare the role of implanting recombinant human bone morphogenetic protein-2 （rhBMP-2） into dog＇s tracheal autograft, allograft and eryo-allograft in inducing regeneration of cartilage. METHODS： The experiment was carried out in the laboratory surgery department of Tangdu Hospital of the Fourth Military Medical University of Chinese PLA between March and October 2004. Twenty-one mongrel dogs were randomly divided into 3 groups： autograft group, allograft group and allograft after cryopreservation group. The four-ring cervical tracheal segment was harvested as the graft and transplanted into the abdominal great omentum. In every allograft, rhBMP-2 with collagen as a cartier was injected into the soft tissue between cartilaginous rings. All the animals were sacrificed in the 5^th postoperative week, the postmortem specimens were examined grossly and histologically, some for measurement of alkaline phosphate activity and calcium content to quantitatively analyze the new cartilage in every specimen. RESULTS： All the dogs survived to the period for being killed. ①Quantitative histological observation： Cartilage rings were harvested after 5 weeks, every experimental group was observed new chondrocytes and cartilage islands. The HPLAS-1000 pathological imaging analytical system calculated new chondrocytes areas were significantly different among the autograft group, allograft group and cryo-allograft group [2 573.6±738.4, 1 691.3±743.6, 2 482.9±827.5, F=5.711, P〈 0.05], and there was no significant difference between the autograft group and cryo-allograft group. ②Detection of alkaline phosphate activity and calcium content： Those in the autograft group and cryo-allograft group were significantly different from those in the allograft group [autograft group ： （7.00±0.50） μkat/g, （5.48±1.15）mg/g; cryo-allograft group： （7.00±0.83） μkat/g, （5.03±0.91）mg/g; allograft group： （5.00±0.67） μkat/g, （3.57±0.98） mg/g, P 〈 0.05], and there was no significant difference between the autogr＇aft group and cryoallograft group （P 〉 0.05）. CONCLUSION： The rhBMP-2 can effective induce new cartilage formation in the tracheal graft, but its effects in tracheal cryo-allograft are similar to those in tracheal autograft, which are obviously superior to those in tracheal allograft.
出处
《中国临床康复》
CSCD
北大核心
2006年第1期91-93,i0006,共4页
Chinese Journal of Clinical Rehabilitation
基金
国家自然科学基金资助(39970707)~~
