摘要
胃蛋白酶消化牛腱胶原的提取纯化以及作为ＢＭＰ的载体复合后注射入小鼠肌肉。术后７天组织学检查可见异位成骨，并有新生血管，胶原部分吸收。２８天时软骨被吸收并被有红骨髓的正常骨组织替代，大部分胶原被吸收。未见排斥反应。钙含量测定显示成骨量大于单纯植入ＢＭＰ组。单纯注射胶原组未见成骨。实验结果表明牛腱胶原经胃蛋白酶消化等提取纯化处理后，抗原性很弱，对部分纯化的ＢＭＰ有助溶助悬作用，吸收快，利于成骨，是ＢＭＰ的一种良好缓释载体。该种胶原与ＢＭＰ的复合物具有较大临床价值。
Pepsindigestedbovinetendoncolagenwaspurifiedandinjectedasasustainedreleasecarrierforbonemorphogeneticprotein(BMP)inthethighofthemouse.Histologicexamination,at7dayspost-implantation,showedectopicboneformationandcapilaryformation.Andthecolagenwasabsorbedpartialy.Thecartilagewasresorbedandreplacedbynormalbonecontaininghematopoieticbonemarrow28daysafterimplantationandthecolagenalmostcompletelydisappearedatthesametime.Noimmunerejectionwasseen.CalciumcontentmeasurementindicatedmorenewboneformationthantheBMPalone.TheseresultsdemonstratedthatthecolagenisefectiveasacarrierofBMP,withtheadvantageofkeepingthelaterstableinthecolagensolution,enhancingthenewboneyieldinduc-tionbyBMPandbeingrapidlybiodegradedbyhosttissues.Apparentlythiscompostitemaybeofvalueinorthopaedicsurgery.
出处
《中华骨科杂志》
CSCD
北大核心
1996年第1期53-55,共3页
Chinese Journal of Orthopaedics
