摘要
本文利用ＭＴＴ法和细胞集落形成抑制试验观察了链球菌７２２对Ｂ１６黑色素瘤细胞体内外生长的抑制作用。结果表明：在０．２ＫＥ／ｍｌ以上浓度的７２２直接作用下，Ｂ１６细胞的活细胞数明显减少（Ｐ＜０．０１），集落形成能力丧失，生长曲线平缓，作用随７２２浓度的增加而增强。提示７２２在体外能抑制Ｂ１６细胞的增殖和生长。动物实验也显示了７２２能抑制Ｂ１６瘤的生长，延长荷瘤鼠的生存时间（Ｐ＜０．０１）。作者认为，７２２制剂抗瘤作用是有效的，但有一定限度，应与手术等方法联合应用，方能取得较好的效果。
he effect of strcptococcal preparation 722 on B16 melanoma cells in vitro is studied,Whcn the B16 cells contactcd with strcptococcal preparation 722 in 0.2-0.5KE/ml concentration directly and continuously in vitro.the percentage of living cells was low, the ability of colony-forming of the cells was lost and the growing-curve was down too.In addition,mices injected with 722 could survive much longer than those not be used with 722.All the results indicated that the effect of 722 preparation on antitumor include the direct effect and the indizect one through stimulating the host's immunologic response ability.
出处
《口腔颌面外科杂志》
CAS
1996年第2期112-114,共3页
Journal of Oral and Maxillofacial Surgery
关键词
黑色素瘤
链球菌制剂
免疫调节剂
Strcptococcal preparation 722 B16 cell Immunotherapy
