摘要
研究了斑地锦总黄酮的提取工艺。结果表明:连续回流法明显优于浸渍法;正交实验得出总黄酮最佳提取条件为A1B3C3D3,即65%的乙醇12倍量提取4次,每次2 h条件下提取液中总黄酮含量最高;各因素对试验结果的影响顺序为D(提取次数)>B(提取时间)>C(加倍量)>A(乙醇浓度)。斑地锦总黄酮是主要的抑菌成分之一,其对温和气单胞菌的最低抑菌浓度为1.25 mg/mL,最小杀菌浓度为10 mg/mL。
An orthogoual experiment [ L9（3^4） ] was carried out to explore the optimum extractive technology for the total flavonoids in Herba Euphorbiae Maculatae. The 4 factors, each with 3 levels, were alcohol concentration, extractive time, solvent volume and extraction time. The optimum combination of the conditions turned out to be 12 -fold volume of 65% alcohol ＋ reflux extraction for 2 h ＋ extraction 4 times, The result of bacteriostatsis was MIC 1.25 mg/mL and MBC 10 mg/mL.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2005年第6期902-905,共4页
Journal of Southwest Agricultural University
基金
重庆市科学技术委员会<防治鱼类细菌性败血病特效中草药及疫苗的研究>资助项目(6849)
关键词
斑地锦
总黄酮
提取工艺
抑菌作用
Herbu Euphorbuae Maculatae. Herha Euphorbiae Maculatae
total flavonoids
extractive, technology
bacteriostasis
