摘要
采用Ｃｙｔｏｐｏｒｅ多孔微载体和低血清添加剂，在１．５ＬＣｅｌｉｇｅｎ灌流培养系统中成功地培养了重组ＣＨＯ工程细胞ＣＬ－１１Ｇ和产尿激酶原单抗的杂交瘤细胞。细胞密度均超过１×ｌ０７／ｍｌ，尿激酶原和抗体的产量均随细胞的密度增加而增加。
Using porous microcarrier Cytopore and low serum medium supplement, a recombinant CHO cell line CL 11G and two strains of hybridomas producing prourokinase monoclonal antibody have been successfully cultivated. The density of these cells could exceed 1×10 7 cells/ml. The yields of prourokinase and monoclonal antibody were enhanced with increasing of cell density. So this porous microcarrier was suitable for large scale cultivation of recombinant CHO cells and hybridomas.
出处
《军事医学科学院院刊》
CSCD
北大核心
1996年第3期191-194,共4页
Bulletin of the Academy of Military Medical Sciences
关键词
CHO细胞
杂交瘤细胞
尿激酶原
单克隆抗体
porous microcarrier
recombinant CHO cell line
hybridomas
prourokinase
antibodies
monodonal
