摘要
rβ-13 CHO 工程细胞在 MC-1型微载体上可良好地附着和增殖,当载体浓度为10mg/ml 时,细胞密度可高达4.5×10~6细胞/ml。由于该细胞具有可自动从载体解离,并再附着和增殖于新载体表面的特性,故采用直接加入新鲜载体和培养基的方法,即可方便地扩大生产。这些结果表明用微载体悬浮培养系统生产 CHO 工程细胞产品将是可行的。
γβ-13 GHO cell line,which was cotransfected with recombinant plasmid PSVEβ inc- luded HuIFN-β gene and PSV_2-dhfr plasmid,was cultivated with M C-1 microcarrier culture system to high density.The cell density could be enhanced by increasing concentration of microcarrier.When concentration of microcarrier was 10 mg/ml,the cell density could reach 4.5×10~6 cell/ml,These experiments showed that this cell line has the property of being re- leased from microcarrier on its own accord and reattached,proliferated on fresh microcarriers. So we successfully scaled up the cultivation with a simple method,i.e.to add the fresh mic- rocarriers and medium directly into the culture system about 2,4 or 8 times of its original volume. These experiments suggested that microcarrier culture system was a very suitable one for producing large quantity,high quality and low cost products by genetically-engineered CHO cell lines.
出处
《军事医学科学院院刊》
CSCD
北大核心
1991年第1期45-50,共6页
Bulletin of the Academy of Military Medical Sciences
关键词
微载体
CHO细胞
培养
microcarrier culture system
rβ-13 CHO cell line
high-density cultivation
