摘要
以重组尿激酶原 (pro UK)为研究对象 ,构建了细胞表达载体 ,对不同细胞表达载体进行了较为系统的比较性研究。确定了所构建pro UK三种载体的表达特性 ,成功建立了pro UK哺乳动物细胞表达系统。重组Namal wa、Vero和Sp2 / 0细胞表达pro UK的水平分别是 2 0 0、12 5和 5 0IU/ (10 6cell.2 4h)。亲和层析纯化 pro UK纯度在 90 %以上。免疫吸附溶酰胺测定表明CHO细胞表达 pro UK单链比例最低 ,而Vero和Namalwa细胞表达 pro UK单链比例最高。该研究对生产 pro UK时 。
Comparison studies of recombinant prourokinase(pro\|UK) in various host cells,and expression vectors were carried out.Expression levels of vectors constructed in this study in different cell lines were compared.Mammalian cells expressing pro\|UK were established.The levels of pro\|UK expression in recombinant Namalwa,Vero and Sp2/0 cells are 200,12\^5 and 50 IU/(10 6 cells 24h).pro\|UK purities separated by immunoaffinity chromatograph are above 90%.Immunoabsorbent assay showed the ratio of pro\|UK in CHO cells is lower than that from Vero and Namalwa cells.This study provide new host cells for pro\|UK production.
出处
《生物工程学报》
CAS
CSCD
北大核心
2000年第3期349-352,共4页
Chinese Journal of Biotechnology
基金
国家高技术研究发展计划项目资助!( 94110 61)
