摘要
目的体外构建组织工程化人工血管模型。方法将诱导细胞制成细胞ECMgel悬液培养,镜下观察细胞形态;将诱导的平滑肌细胞和内皮细胞悬液分层种植于支架内表面,以荧光显微镜、扫描电镜和HE染色光镜观察。结果在ECMgel凝胶中细胞生长良好;旋转培养的血管模型,质地均匀,内腔面光滑;HE染色可见血管壁三层结构:内皮细胞层、平滑肌细胞层、PGA+交联胶原层;扫描电镜可见内皮细胞融合成片;荧光显微镜观察可见均匀分布的内皮细胞。结论①ECMgel是携带细胞良好的基质;②旋转培养条件可以促进细胞的贴附和分化;③利用体外诱导的种子细胞分层种植可以构建成与天然血管结构相似的血管模型。
Objective To create a tubular model of tissue engineering vessel made of vascular smooth muscle-like cells （VSMLCs） and vascular endothelium-like cells （VELCs） through dynamic rotation culture technique. Methods VSMLCs were resuspended by ECMgel solution and a tri-dimensional cell culture system was developed. VSMLCs suspension was delivered regularly into the luminal surface of tubular scaffold with slow rotation. The tubular scaffold with VSCLCs was poured with the solution of VELCs. The characteristics of vessel model was analyzed in scanning electron microscope, fluorescence microscope and light microscope. Results Distinct layered VSMLCs appeared in ECMgel. Vessel model had smooth inner surface, still elasticity and tenacity;The histological sections showed synthetic artery architecture resembling the natural artery had consisted of three layers. Confluent mono-layer had been composed of VECLs labeled by DAPI. Conclusions ①ECMgel is a good matrix for VSMLCs to adhere and grow;②Rotation culture technique that facilitate cells＇ attachment and differentiation;③A tubular laminated model of tissue engineering vessel, resembling the natural artery, can be created with bone marrow-derived mesenchymal stem cells.
出处
《基础医学与临床》
CSCD
北大核心
2006年第2期192-196,共5页
Basic and Clinical Medicine
关键词
小口径血管
种子细胞
血管模型
旋转培养
small-diameter vessel
seeded cells
vessel model
rotation culture
