摘要
目的:结合巯基棉分离富集方法,建立一种测定水中痕量铜的共振光散射(RLS)新方法.方法:用简易荧光计研究在碱性介质中,铜-硫化钠-十六烷基三甲基溴化铵体系的共振散射光谱,考查了它们的光谱特征,影响因素和适宜的反应条件.结果:在实验条件下,RLS增强程度与Cu2+的浓度在 0.25～3.50 μg/ml 范围内具有良好的线性关系(r=0.9992),方法检出限 0.017 μg/ml.结论:该法在室温下进行,操作方便,具有较高的灵敏度;可用于环境水样中铜浓度的测定.
Objective：To set up a resonance light scattering method for determination of trace copper in water sample with the sulfhydryl cotton separation - enrichment method. Methods： The resonance light scattering spectrum of cetyltrimethyl ammonium bromide in alkaline medium was enhanced significantly in the presence of CuS/S^2- solution. The spectral characteristics, the affecting factors and the optimum conditions of the reaction were thus investigated by easy fluorophotometer. Results： On the experimental conditions, the enhancement of resonance light scattering at 440 nm was proportional to the concentration of Cu（Ⅱ） rangeing from 0. 25 to 3.50 μg/ml（r =0. 9992） ,and detection limit was 0. 017 μg/ml. Conclusion：This method can be operated conveniently at ambient temperature with high sensitivity and can be used to determine Cu（Ⅱ） in the environmental water sample.
出处
《中国卫生检验杂志》
CAS
2005年第11期1286-1287,共2页
Chinese Journal of Health Laboratory Technology
基金
江西省自然科学基金项目(0320039)
关键词
铜
十六烷基三甲基溴化铵
共振光散射
Copper（Ⅱ）
Cetyltrimethyl ammonium bromide
Resonance light scattering
