摘要
目的:研究金纳米棒(GNRs)IgG生物学标记及其在抗人IgG检测中的应用。方法:利用种子生长法制备GNRs,用巯基十一酸(MUA)对GNRs端头邀111妖晶面进行化学修饰,MUA提供的羧基可与人IgG结合;抗人IgG与活化的GNRs反应引起GNRs表面等离子体共振(SPR)特征变化,通过读取SPR值判断免疫反应的结果。结果:合成了不同长径比(AR)的GNRs,成功地将人IgG标记于GNRs(AR=3.7)的端头;利用标记后的GNRs对抗人IgG进行检测,其SPR最大吸收峰发生9nm红移,检测灵敏度可达纳摩尔量级。结论:基于人IgG-抗人IgG免疫反应建立了GNRs用于免疫检测的方法,为GNRs用于免疫检测进而研制免疫传感器奠定了基础。
Objective： To establish the method of labeling gold nanorods（GNRs） with human IgG and apply it in detecting anti-human IgG. Methods： GNRs were produced by a seed-mediated growth method. Human IgG was immobilized on the {111} end faces of GNRs through 11-mercapto-undecanoic acid containing a terminal carboxyl group. The surface plasmon resonance（SPR） band of GNRs was changed by attracting biological receptor molecules anti IgG. Results： GNRs labeled with human IgG was successfully prepared. The detections for target protein anti-IgG by GNRs （aspect ratio=3.7） binding human IgG led to a red shift for 9 nm. The sensitivity of this method can reach nanomole of protein. Conclusion： The method of GNRs used in immunoassay was established and it helps to develop the GNRs immno-biosensor.
出处
《生物技术通讯》
CAS
2009年第5期680-682,702,共4页
Letters in Biotechnology
基金
国家高技术研究发展计划(2006AA03Z326)
