摘要
目的为了研究Ⅰ型白介素Ⅰ受体(IL-1Rt1)基因表达与狼疮性肾炎进展的关系。方法采用32P同位素标记cDNA探针狭线印迹杂交方法,检测自发狼疮性肾炎的BXSB小鼠肾脏组织中IL-1Rt1基因mRNA的转录水平。结果发现与狼疮性肾炎发病较轻的3月龄雄性BXSB小鼠相比较,狼疮性肾炎发病较重的6月龄雄性BXSB小鼠肾脏组织表达IL-1Rt1 mRNA的量显著增加(P<0.05)。结论IL-1Rt1 mRNA的异位大量表达是狼疮性肾炎肾小球细胞外基质病理性积聚的重要原因,可能会引起肾小球结构和功能多方面的改变,在狼疮性肾炎的进展和肾小球硬化的形成过程中起重要作用。
Objective： BXSB mice spontaneously develop a progressive and lethal lupus nephritis which is regarded as an experimental mouse model for human systemic lupus erythematosus（SLE）. The etiopathogenisis of SLE in these mouse remain poorly defined. In order to study the role of interleukin 1 recept type 1 （IL- 1Rt1） genes expression in lupus nephritis progression. Methods：Transcription of IL- 1Rt1 mRNA in the kidneys of BXSB mice was detected by using slot blot hybridization with^32p isotope labeled cDNA probe. Total RNA was isolated from kidneys of 6 month old male and 3 month old male BXSB mice using a single- step method by acid guanidnum thiocyanate phenol chloroform extraction. Results： The 6 month old BXSB male mice with very active diseaes expressed significantly elevated concentration of IL- 1Rt1 mRNA when compared with 3 month old male BXSB mice as controls（ P 〈 0. 05）. Conclusion：These results suggest that increased expression of IL- 1 Rt1 mRNA may alter glomerular structure and function, and may thereby play a important role in the glomerular accumulation of pathological extraceUular matrix in lupus nephritis and contribute to lupus progression and glomerulosclerosis in these SLE model.
出处
《皮肤病与性病》
2005年第3期1-2,共2页
Dermatology and Venereology
关键词
红斑狼疮
系统性
狼疮肾炎
白介素Ⅰ
Lupus erythematosus
systemic
lupus nephritis
Interleukin 1.
