摘要
目的 :观察中药狼疮静颗粒对狼疮性 BXSB小鼠肾脏免疫组化的影响。方法 :取 40只 (♀8只 , 32只 ) BXSB小鼠 ,8♀ BXSB小鼠作为同种阴性对照组 ,其余 32只 BXSB小鼠随机分为模型对照组、中药组 ,CTX组、CTX+中药组 ,每组各 8只。各组于用药 2周后取肾脏进行冰冻切片 ,并用直接免疫荧光染色法 ,在荧光显微镜下观察各组肾脏 Ig G荧光染色阳性肾小球数、荧光染色强度、荧光染色面积。结果 :模型对照组绝大多数肾小球毛细血管襻上可见大量 Ig G呈片状沉积 ,沉积范围累及整个肾小球 ,且荧光强度高 ;经过治疗后 ,中药组荧光染色阳性肾小球数 /肾小球总数比值明显降低 ,荧光强度明显减弱 ,累及肾小球荧光染色面积明显减小 ,分别与模型对照组比较 ,差异均具有显著性意义 ( P<0 .0 1 )。结论 :中药狼疮静颗粒具有明显清除狼疮性 BXSB小鼠肾脏 Ig G沉积的作用。
Objective: To investigate the effect of Lang-Chuang-Jing on the kedney immunohistochemistry of lupus-BXSB-mice.Methods:The number,intention and area of positive glomerular IgG were observed on renal frozen slices with direct immunofluorescence.Results: A large number of patchy sedimention og IgG with high intention all over the glomerulus could be observed on the most of glomerular capillary loop in the model control group.After treatment,the ratio,intention and area of psitive fluorescence glomerulus in the treatment group were obviously reduced.And all those between treatment and control group were significantly differently(P<0.01). Conclusion:The traditional Chinese medical decoction of Lang-Chuang-Jing has a significant effect on eliminating IgG sedimentation in lupus BXSB mice' glomerulus.
出处
《浙江中西医结合杂志》
2003年第2期71-73,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
