摘要
比较山梨醇和蔗糖两种碳源在苹果微体繁殖中的作用表明，二者对增殖和生长的最适浓度均为３０ｇ／Ｌ．山梨醇代替蔗糖作碳源时芽的增殖倍数高，而新梢生长差，叶／茎值小。总碳源为３０ｇ／Ｌ时，采用不同比例的山梨醇和蔗糖配合有利于芽的增殖和生长。山梨醇对生根的作用显著低于蔗糖，而在山梨醇培养基上继代４次后。
The influences of two carbon source,sorbitol and sucrose,in micropropa-gation of apple were compared.The results showed that the optimal concentration ofboth sorbitol and sucrose was 30 g/L for shoot proliferation and growth.The substitu-tion of sorbitol for sucrose could promote proliferation,but could decrease shoot growthleaf/stem ratio,A medium with various proportion with total carbon source of 30 g/L ofsorbitol and suerose favored both proliferation and shoot growth.Rooting was worse onsorbitol medium than on sucrose one but would be greatly enhanced after subcultured 4times.
出处
《西北农业大学学报》
CSCD
1996年第4期102-104,共3页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
关键词
苹果
山梨醇
微体繁殖
增殖
生根
apple,sorbitol,micropropagation,proliferation,rooting
