摘要
目的观察细胞外基质金属蛋白酶诱导剂(EMMPRIN/CD147)对类风湿关节炎(RA)患者滑膜成纤维细胞(FLS)合成基质金属蛋白酶(MMPs)的作用。方法手术切除的RA患者的滑膜组织,分离成FLS,传代培养。明胶酶谱法测FLS的MMPs含量。将FLS与高表达EMMPRIN/CD147的单核细胞株(THP)-1共培养,观察THP-1细胞对FLS MMPs表达量的影响,同时观察EMMPRIN/CD147拮抗肽激活蛋白(AP)-9对FLS MMPs表达的影响。结果THP-1细胞表达高水平的CD147,而MMP-2、MMP-9的表达水平很低;分离的FLS表达低水平的CD147和一定水平的MMP-2、MMP-9。二者混合培养后,FLS表达MMP-2、MMP-9水平显著升高,随着THP-1细胞数的增加,MMP-2、MMP-9的合成量也增加。THP-1对FLS合成MMP-2、MMP-9的刺激作用可被CD147拮抗肽AP-9所抑制。结论CD147对RA患者FLS合成MMP-2、MMP-9有刺激作用,而这种刺激作用可被CD147拮抗肽AP-9所抑制。
Objective To study the stimulating effect of extraeellular martrix metalloproteinase iudueer （EMMPRIN/CD147） on the production of matrix metalloproteinases （MMPs） by fihrohlasts isolated from rheumatoid arthritis （RA） patients. Methods Fibroblasts were isolated from svnovial tissues reseeted from rheumatoid arthritis patients during svnoveetomy, and were confirmed by CD14. CD68 detection by flow eytometry （FCM） and vimentin protein by immunostaining （SP）. The synovial fibroblasts （FLS） were co-cultured with THP-1 cell （a eell line of monocytes）. The produetion of matrix metallnproteinases （MMPs） by synovial fibroblasts were evalnated by Gelatin zymngraphy and RT-PCR. The expression of CD147 on THP-1 cells and synovial fibrohlasts were detected by FCM. Antagonist peptide to EMMPRIN/CD147 AP-9 was used to study the effeets on MMPs production. Results The isolated synovial fibroblasts were eharaeterized by negative CD14,CD68 and positive vinlentin protein, and expressed low level of CD147. and high levels of MMP-2, MMP-9. While THP-1 cells expressed very high levels CD147 and low level of MMP-2. MMP-9. When synovial fibroblasts were cocnltured with THP-1. the production of MMP-2, MMP-9 was greatly enhanced by FLS and suppressed bv the antagonist peptide （AP-9） of CD147. Conclusion CD147 can enhance the production of MMP-2, MMP-9 hy synovial fihrohlasts.
出处
《中华风湿病学杂志》
CAS
CSCD
2005年第9期537-540,共4页
Chinese Journal of Rheumatology
基金
国家863基金资助项目(2001AA215061)
