摘要
目的 为了探讨关节内CD14 7表达细胞的来源与类风湿关节炎 (RA)的关系 ,研究CD14 7分子在RA患者及正常健康人外周血与滑液细胞中的表达水平。方法 取 10例活动期RA患者用药治疗前后外周血、治疗前滑液及 10名正常健康对照外周血 ,外周血和滑液分离后细胞悬液采用双色免疫荧光标记 ,流式细胞术测量淋巴细胞 (CD3+ T)、CD14 + 单核细胞和中性粒细胞表达CD14 7阳性率及表达荧光强度水平。结果 ①与正常对照相比 ,RA患者外周血CD3+ T淋巴细胞与CD14 + 单核细胞的表面CD14 7表达强度较高 (P <0 0 1) ,而其表达率比较差异无显著性。中性粒细胞表面CD14 7的表达率与表达强度与正常人相比差异均无显著性 ;②正常对照外周血细胞膜表面CD14 7的分子的表达在IFN γ刺激后有显著增多 ,而RA患者在刺激前后差异无显著性 ;③关节滑液中淋巴细胞、单核细胞与中性粒细胞上的CD14 7表达强度均比外周血细胞表达高 ,且淋巴细胞的表达率较高。结论 CD14 7在RA中各种炎细胞上均有较高表达 ,提示其在RA的病理损伤中起到重要作用。
Objective To investigate the source of CD147 expressing cells in joint and its corelation with rheumatoid arthritis (RA),and to study the expression of CD147 on different cells of peripheral blood and joint fluid of RA patients and normal health controls.Methods The expression rate and intensity of CD147 on lymphocyte (CD3 +T),monocyte (CD14 + Mo) and neutrophils (N) of 10 active RA patients before and after treatment and 10 normal health controls′ peripheral blood and synovial fluid were studied by bicolor immunofluorescence flow cytometry.Results ① Compared with normal control,the expression intensity of CD147 on CD3 + T and CD14 + Mo were higher (P<0.01)and the expression rate had no significant difference.② IFN-γ increased the expression of CD147 on normal control peripheral cells but had no effect on RA.③The expression intensity of CD147 on synovial fluid cells was higher than that on peripheral cells and lymphocytes had higher expression rate.Conclusion Most of the inflammatory cells in RA express CD147,indicating that CD147 may play an important role in the RA pathology.
出处
《中华风湿病学杂志》
CAS
CSCD
2003年第12期742-745,共4页
Chinese Journal of Rheumatology
基金
国家"8 63"资助项目 ( 2 0 0 1AA2 15 0 61)
关键词
类风湿关节炎
外周血
滑液细胞
CD147分子
表达
Cell adhesion molecules
CD147
Arthritis,rheumatoid
Lymphocytes
Monocytes
Neutrophils
