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PMA诱导的THP-1细胞分化过程中MMP-9及MMP-2的表达与细胞侵蚀性的关系 被引量：3

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摘要 　目的: 应用单核细胞THP -1株建立单核细胞向巨噬细胞分化的体外模型, 并探讨THP- 1细胞分化过程中基质金属蛋白酶 9(MMP -9)及基质金属蛋白酶 2 (MMP -2)的表达与细胞侵蚀性的关系以及巨噬细胞在类风湿关节炎 (RA)发病机制中的作用。方法: 用乙酸肉豆蔻佛波酯 (PMA) 诱导THP- 1细胞向成熟的单核 巨噬细胞分化后, 采用倒置显微镜观察分化细胞的形态学变化。用流式细胞术测定细胞表面特异性抗原CD14的表达。采用明胶酶谱 (gelatin- zymogram)分析法测定分化前后THP- 1细胞中MMP -9和MMP- 2的表达。采用侵蚀性小室 (BoydenChamber -Matrigel人工重组基底膜 )法, 观察分化前后的THP- 1细胞侵蚀性的变化。结果: THP- 1细胞向单核 巨噬细胞分化过程中, 细胞由分散、悬浮转变为黏附、贴壁, 且出现CD14的高表达。分化后的THP- 1细胞中MMP -9和MMP- 2的表达明显增多, 侵蚀性明显增强。结论:建立了THP- 1单核细胞的体外分化模型。该细胞在分化过程中, MMP- 9和MMP -2的表达增多、侵蚀性增强, 为巨噬细胞在RA发病机制的作用提供了一定的实验依据。
作者 周筠 朱平 蒋建利 陈志南 姚西英 贾俊峰 樊春梅
机构地区 第四军医大学西京医院临床免疫科 西安交通大学医学院药理教研室 第四军医大学基础部细胞工程研究中心
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2005年第2期256-257,260,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 国家高技术研究发展计划 ( 863 )主题重点项目 (No.2001AA215061)
关键词 单核细胞 乙酸肉豆蔻佛波醇 细胞分化 基质金属蛋白酶 侵蚀性
分类号 R329.26 [医药卫生—人体解剖和组织胚胎学]
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参考文献9

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二级参考文献7

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共引文献13

同被引文献26

  • 1王梅芳,杨林花.MMP-9与冠状动脉粥样硬化[J].临床心血管病杂志,2004,20(11):699-701. 被引量：8
  • 2吕运成,王佐,危当恒,姜志胜,万炜,李国华,童中艺,王贵学.基质细胞衍生因子1α—CXCR4趋化THP-1细胞迁移及氧化型低密度脂蛋白的影响[J].中国动脉硬化杂志,2007,15(1):15-18. 被引量：6
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细胞与分子免疫学杂志
2005年 第2期

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