摘要
目的研究同型半胱氨酸(HCY)、同型半胱氨酸诱导基因-2抗体(HCY-2Ab)及叶酸对人胚心肌细胞超微结构的影响。方法取培养的人胚心肌细胞,进行HE染色,常规电镜包埋,透射电镜观察。结果当HCY的浓度小于1·0mmol/L时,随着HCY浓度的升高,心肌细胞核内常染色质增多、细胞质内线粒体和粗面内质网均多于空白对照组,而糖原颗粒减少;当浓度超过1·0mmol/L时,心肌细胞逐渐退化,细胞核内常染色质减少,异染色质增多,核膜包裹染色质形成凋亡小体,细胞质内线粒体肿胀、数目减少,粗面内质网减少;加入外源性的HCY-2Ab或叶酸,心肌细胞形态较相同HCY浓度下的好转。结论HCY对培养的人胚心肌细胞超微结构的维持具有正调节和负调节双相作用;外源性的HCY-2Ab和叶酸在一定程度上可以逆转HCY的作用。
Objective To study the effects of homocysteine （ HCY）, homocysteine induced gene-2 antibody （ HCY-2 Ab） and folic acid on the ultrastructure of human fetal cardiomyocytes. Methods Utilizing the cultured human fetal cardiomyocytes, HE staining and electron microscope were used to study the structure and ultrastructure of the cardiomyocytes with normal means. Results When the concentration of HCY ranged from 0 to 1.0 mmol/L, the shapes and ultrastructure of the cultured cardiomyocytes were not changed and the function became active gradually;they degenerated gradually when the concentration exceeded 1.0 mmol/L; the morphological characters of the cardiomyocytes were much better when HCY-2 Ab or folic acid was added to the culture medium than that at the same concentration of HCY. Conclusion HCY plays dual roles on the shapes and the function of human fetal cardiomyocytes. The addition of exogenous folic acid or HCY-2 Ab can reverse the effects that HCY treatments have on cardiomvocvtes in a certain degree.
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第4期428-432,共5页
Acta Anatomica Sinica
基金
北京大学人类疾病基因研究中心资助项目(2000-A-4)
