摘要
目的 研究同型半胱氨酸诱导基因 (HCY 2 )在发育不同时期的正常人胚和畸形人胚心脏中的表达 ,揭示HCY 2表达蛋白在人胚心肌细胞发育和分化中的作用。 方法 取正常和先天性畸形人胚心脏 ,采用免疫组织化学及图像分析技术对心脏内HCY 2表达变化进行定量测定分析。 结果 HCY 2在人胚心脏发育整个时期均表达 ,其表达部位主要在心肌纤维内 ;先天性畸形人胚心脏中的HCY 2表达增强。 结论 HCY 2表达蛋白通过影响心肌细胞的增殖而影响人胚心脏发育过程 ,其表达异常与先天性畸形的发生密切相关。
Objective To study the expression of homocysteine induced gene(HCY-2) in the normal and abnormal human embryonic heart. To research the roles of HCY-2 expressing protein in the human embryonic ventricular myocardial cell development and differentiation. Methods Immunohistochemistry and the technique of picture assay were used to detect HCY-2 expression in the normal and abnormal human embryonic heart. Results HCY-2 expresses during the development of human embryonic heart and mainly in the myocardial cells.The expression increases in the congenital malformation.Conclusion HCY-2 expression protein affects the development of human embryonic heart through affecting the myocardial cells proliferation and the abnormal expression has close relation to the congenital malformation.
出处
《解剖学报》
CAS
CSCD
北大核心
2004年第1期96-99,共4页
Acta Anatomica Sinica
基金
北京大学人类疾病基因研究中心资助项目 ( 2 0 0 0A 4)
