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多辐溲疏群体同工酶变异的数量分析 被引量:11

NUMERICAL ANALYSES OF ISOZYME VARIATION WITHIN AND AMONG THE POPULATIONS OF DEUTZIA MULTIRADIATA
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摘要 研究了四川南川金佛山特产的多辐溲疏(Dentziamultiradiata)不同海拔和生境的5个群体的41个个体叶片过氧化物酶、过氧化氢酶、酯酶、淀粉酶、柠檬酸脱氢酶及谷氨酸脱氢酶同工酶谱带位置上的变异式样。来自同一群体的不同个体在谱带上表现出极大的相似性,而来源于不同群体的个体却表现出明显的趋异性,显示出酶谱带位置与群体生长的生境有显著的相关性,一定的群体具有某些特有的酶谱带。空间隔离限制了群体间个体的相互基因交流,在进化中那些适应新的生境的遗传变异体得以保存下来,致使群体间在酶谱上愈来愈不同。而同一群体内植株间通过异花传粉虽能在个体间实现频繁的基因交流而增加其遗传变异性,但这种遗传变异性多数不能通过种子繁殖方式固定下来。南川金佛山目前虽能为该种提供少数岛屿状的适宜生境,但如果这些生境发生变化,而该种又不能提供新的遗传变异源,则其发展前景是堪忧的。 With the electrophoretic technique, we studied the Peroxidas (PER),Catalase (CAT), Esterase (ES), Amylase (AA), Citric acid dehydrogenase (CDH) and Glutemate dehydrogenase (GDH) in leaves of 41 plants from 5 populations with different altitudes and habitats of Deutzia multiradiata W. T. Wang which is endemic to Mt. Jinfo of Nanchuan, China. The bands of those isozymes are scored to construct the two-state data matrix which is caculated by Jaccard, Sobal-Sneath and Sobal-Michener association coefficients clustered with UPGMA and WPGMA respectively. The result reveals that individuals from the same populatin are highly homogenous genetically interprated from bands of the six isozymes, while individuals from the different populations show highly dissimilarities in isozyme bands. It is clear that the isozyme bands of a given population are notably correlated with the habitats from which it was collected. Certain populations possess some unique isozyme bands, which can be explained by the distributive pattern and the reproductive features of the species. It distributes as scattered populations which are isolated each other spatially. Such spatial isolation largely inhabites the gene exchange among individuls from different populations and those adapted genetic variations to these habitats are selected and remained in the volutionary process, which makes the genetic divergence among populations more obvious. The habitats here only play the role in selecting and fixing the genetic variations of populations adapted to that habitat. Though the remarkable hybridizations among individuals of the same population lead to more easily gene exchange and can enrich the genetic heterogeneity within the same populaton, the transferring of those rich genetic variation within the same population by means of seeds to their offsprings is nearly impossible because the species are allogamy and or xenogamy but most of the seeds it produced are abortive. The vegetative reproduction of the species causes the stronger isozyme homogeneity among individuals within the same population.
作者 何平 谈锋
出处 《云南植物研究》 CSCD 1996年第2期167-175,共9页 Acta Botanica Yunnanica
基金 国家自然科学基金 四川省青年科技基金
关键词 虎耳草科 溲疏属 虎多辐溲疏 植物群体 同功酶 Numerical analyses, Isozyme variation, Genetic differentiation, Population, Deutzia multiradiata
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参考文献9

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