摘要
本文用聚丙烯酰胺凝胶电泳测定了粘虫(Mythirana separata)五个地区群体(福建、贵州、安微、北京和吉林)的同工酶变异。分析10种同工酶(ADH,AO,GOT,α-GPDH,HK,LAP,LDH,MDH,ME和TYR)的14个座位,其中单型座位有8个,多型座位有6个,后者占42.9%。五个地区群体的平均杂合度为0.138。根据14个基因座位的等位基因频率计算出的五个地区群体间的平均遗传相似度为0.978,平均遗传距离为0.023,表明这五个地区群体的基因结构基本相似,遗传分化程度很小。可以认为,在这五个地区群体之间不存在基因流动的障碍。这从遗传和变异的角度证明了粘虫的远距离迁飞特性,迁飞阻止了不同地区群体的遗传分化。
Isozyme variation in populations from five geographical localities (Fukien, Guizhou, An-hui, Jilin and Beijing) of the armyworm Mythimna separata was investigated with polyacryla-mide gel electrophoresis. Fourteen gene loci from 10 enzymes (AO, ADH, EST, GOT, α-GPDH, HK, LOH, MDH, ME and TYR) were analyzed. Of the 14 loci examined, 8 were-monomorphic and 6 were polymorphic with a proportion of 0.429. Among these populations the mean heterozygositp was 0.318, mean genetic identity based on 14 loci was 0.978 and mean, genetic distance was 0.023. This result indicates the limitation of genetic differentiation and it may be concluded that no barrier of gene flowexists among these populations. This is conceivable because the armyworm moths are capable of long distance migration which would hamper genetic differentiation of different local populations.
出处
《昆虫学报》
CAS
CSCD
北大核心
1992年第1期33-39,共7页
Acta Entomologica Sinica
关键词
粘虫
同功酶
Mythimna separata-isozyme