摘要
研究卡那霉素、潮霉素、头孢霉素和羧苄青霉素对剑麻愈伤组织生长、不定芽分化及生根的影响,并确定抑制农杆菌生长的头孢霉素、羧苄青霉素的质量浓度。结果表明:潮霉素明显抑制剑麻愈伤组织生长和不定芽分化,卡那霉素次之,头孢霉素和羧苄青霉素相对较小;4种抗生素均能抑制剑麻生根,其抑制效果依次为头孢霉素>卡那霉素>羧苄青霉素>潮霉素。头孢霉素和羧苄青霉素的浓度大于200 mg/L时,农杆菌生长完全被抑制。在筛选剑麻转化体时,卡那霉素和潮霉素的使用浓度分别以100、50 mg/L为宜,羧苄青霉素和头孢霉素的使用浓度均为200 mg/L。
The dose response assay was performed to insight into the effects of kanamycin, hygromycin, cefotaxime, and carbenicillin on the callus growth, adventitious buds regeneration and root induction of sisal. The optimum concentrations of cefotaxime and carbenicillin inhibiting Agrobacterium was determined. The results showed that the callus growth and adventitious buds regeneration were greatly inhibited by hygromycin, followed by kanamycin, cefotaxime and carbenicillin. Root induction was inhibited by each of the four antibiotics, with the order of cefotaxime 〉 kanamycin 〉 carbenicillin 〉 hygromycin. The growth of Agrobacterium was completely inhibited by 200 mg/L cefotaxime or carbenicillin. In conclusion, 100 mg/L kanamycin, 50 mg/L hygromycin, 200 mg/L cefotaxime or carbenicillin was recommended to screen transformed tissue in Agrobacterium-mediated transformation of sisal.
出处
《热带农业科学》
2013年第1期5-10,共6页
Chinese Journal of Tropical Agriculture
基金
中央级公益性科研院所基本业务费专项(No.sscri200802
No.1251022011010)
现代农业产业技术体系建设专项资金资助(No.CARS-19)
关键词
剑麻
抗生素
愈伤生长
植株再生
sisal
antibiotic
callus growth, plant regeneration
