摘要
[目的]建立虎舌红的高频再生体系，为利用转基因技术改良其品质奠定基础。[方法]以虎舌红试管苗顶端叶片为试材进行组织培养和不定梢诱导试验。[结果]外植体在9种培养基上均能诱导出愈伤组织，4号培养基的不定芽再生率最高，为45．80％，植株生长健壮；其次是7号培养基，其不定芽再生率为39．76％。从整体上看，不定芽的再生率都不高，最高的也未超过50．00％。综合考虑愈伤组织诱导和不定芽再生，适合虎舌红叶片不定芽再生的培养基为：1／2 MS＋TDZ 2．0mg／L＋IAA0．2～0．4mg／L＋AgNO3 3．0～5．0mg／L。选健壮苗接种于1／2MS＋IBA 0．3mg／L＋NAA 0．5mg／L培养基上，20d后生根率可达90％，炼苗后再生植株的移栽成活率达80％以上。[结论]该研究成功建立了虎舌红叶片的离体再生体系。
The aim of the study was to establish the high-frequency regeneration system of Ardisia mamillata and lay a basis for improving its quality by transgenic technology. [ Method] With the apical leaves of tube A. mamillata seedlings as tested materials, its tissue culture and induction experiment of adventitious shoots were performed. [Result] The ealli could be induced from all the explants growing on the 9 media. On Medium 4, the regeneration rate of adventitious buds was highest, being 45.80% and the plantlets grew mbnstly. On Medium 7, the regeneration rate of adventitious buds was secondary, being 39.76%. Totally, the regeneration rate of adventitious buds was not high and the highest did not exceed 50.00%. Considering callns induction and the regeneration of adventitious buds comprehensively, the suitable medium for the regeneration of adventitious buds from A. mamiliata leaves was 1/2 MS ＋ TDZ 2.0 mg/L ＋ IAA 0.2-0.4 mg/L ＋ AgNO3 3.0- 5.0 mg/L. The strong cultured seedlings were selected and inoculated onto the medium 1/2 MS ＋ IBA 0.3 mg/L ＋ NAA 0.5 mg/L, their rooting rate could reach 90% after 20 d and the transplanting survival rate of hardened regenerated plants was higher than 80%. [ Conclusion] The in vitro regeneration system of A. mamillata leaves was estabished successfully in this study.
出处
《安徽农业科学》
CAS
北大核心
2009年第17期7854-7854,7874,共2页
Journal of Anhui Agricultural Sciences
基金
江西省教育厅科技项目(GJJ09603)
关键词
虎舌红
叶片
不定梢再生
Ardisia mamillata
Leaves
Regeneration of adventitious shoots
