摘要
目的:考察不同提取方法对胡芦巴碱提取率的影响,建立胡芦巴中胡芦巴碱含量测定的高效液相色谱方法.方法:分别以水、甲醇、无水乙醇及三种比例的甲醇-水为溶剂,用超声等方法考察提取率.HPLC测定采用Zorbax XDB-C18分析柱,流动相为0.37mmol·L-1磷酸溶液(pH=3.55),检测波长265nm.结果:以甲醇-水(1:1)超声提取40min胡芦巴碱的提取率较高;样品中胡芦巴碱进样量在0.40～3.96μg范围内线性关系良好,r=0.9999(n=5),加样回收率98.18%,RSD=1.54%(n=6);33份胡芦巴商品药材中胡芦巴碱的含量为0.243%～0.731%,超过文献报道的胡芦巴碱含量1倍以上.结论:提取及含测方法操作简便,结果可靠,可为胡芦巴质量控制提供科学依据.
Objective ： To develop a method for the extraction and determination of trigonelline in Huluba （seeds of Trigonella foenum-graecum L. ）and compare the effect of different extraction methods by determination of trigonelline. Metbod：Huluba sample was extracted by ultrasonic cleaner（500W）and other methods in methanol,absolute ethanol,water and different concentrations of methanol-water,respectively. The experimental conditions were as follows,the Angilent Zorbax Eclipse XDB- C18 column,mobile phase 0. 37 mmol · L^-1 phosphoric acid （pH= 3.55）,flow rate 1 ml· min^-1 ,and detection wavelength UV 265nm. Results：In the extraction with ultrasonic cleaner （500W） in methanol-water（1 ： 1）,higher efficiency was showed. The standard curve was linear in the range of 0.40- 3.96μg ,r = 0. 9999 （n = 5）. The recovery rate was 98.18 %, RSD： 1.54 % （n= 6 ）. The result of determination of trigonelline in 33 comercial samples of Huluba was 0. 243 % -0.731% which was more than two times its value reported in literatures. Conclusion：A method for extraction and determination of trigonelline was established ,which was efficient ,simple and reliable ,and can be used for the quality control of Huluba（fenugreek）.
出处
《中国药品标准》
CAS
2005年第4期11-14,共4页
Drug Standards of China
