摘要
目的 :建立高效液相色谱法测定胡芦巴中胡芦巴碱的方法。方法 :石油醚 无水乙醇提取 ,固定相氨基键合相色谱柱 ,流动相乙腈 水 (75∶2 5 ) ,检测波长 2 6 5nm。结果 :进样量 3.6 8～ 73.6 0 μg·mL-1,r =0 .9999,加样回收率 97.4% ,RSD 1.83% (n =6 )。结论 :可为胡芦巴的质量控制提供依据。
Objective:A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum .Method:The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH 2P-50 column was used,mobilephase consisted of acetonitrile-water(75∶25) and detection wavelength was set at UV 265 nm.Result:The standard curve was linear in the range of 3.68～73.60 μg·mL -1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4 %and 1.83%( n =6) respectively.Conclusion:It provides scientific indexes for quality control of T. foenum-graecum .
出处
《中国中药杂志》
CAS
CSCD
北大核心
2002年第3期194-196,共3页
China Journal of Chinese Materia Medica
