摘要
建立了亲水作用色谱法(HILIC)测定胡芦巴药材中胡芦巴碱含量的方法。采用Waters Atlantis HILICS ilica色谱柱(150mm×2.1mm,3μm),以乙腈-乙酸铵溶液(pH4.4)(体积比为70:30)为流动相,流速0.4mL/min,检测波长265nm。胡芦巴碱的线性范围为2.50～100mg/L(r=0.9996);两个加标水平的平均加样回收率为102%,相对标准偏差(RSD)分别为4.17%和2.28%(n=3)。结果表明所建方法分离效果好、快速简易,可以弥补中国药典中离子对色谱法(IPLC)平衡时间过长的缺陷,适用于胡芦巴药材中强极性胡芦巴碱的测定,为胡芦巴的质量控制提供了有效的方法。
A method of hydrophilic interaction chromatography （HILIC） was established for the quantitative determination of trigonelline in Trigonella foenum-graecum L.HILIC analysis was performed on a Waters Atlantis HILIC Silica column （150 mm×2.1 mm,3 μm）.The mobile phase consisted of acetonitrile-ammonium acetate （pH 4.4） （70∶30,v/v）,and the flow rate was 0.4 mL/min.The detection wavelength was set at 265 nm.The method has good linearity in the range of 2.50-100 mg/L for trigonelline （r=0.9996）.The recoveries were on an average of 102%by adding 29.2 mg/L and 43.8 mg/L with relative standard deviations （RSDs） of 4.17% and 2.28% （n=3）,respectively.The results indicate that the method is simple and rapid for the determination of strong polar trigonelline in Trigonella foenum-graecum L.Furthermore,it significantly reduces the equilibration time compared with ion-pair liquid chromatography （IPLC） recorded in the Pharmacopoeia of China.This new method can be used as a valid method for the quality control of Trigonella foenum-graecum L.
出处
《色谱》
CAS
CSCD
北大核心
2010年第4期379-382,共4页
Chinese Journal of Chromatography
基金
中国科学院重要方向性项目(No.KSCX2-YW-R-078)
科技部创新药物重大专项关键技术(No.2009ZX09502-023)
关键词
亲水作用色谱法
胡芦巴碱
胡芦巴
中药材
hydrophilic interaction chromatography （HILIC）
trigonelline
Trigonella foenum-graecum L.
traditional Chinese medicine （TCM）
