摘要
用常规诱变育种方法对西索米星小单孢菌进行诱变育种，获得ＮＳ８－３０菌株，结合发酵条件研究，发酵效价比原始菌株提高１７倍以上，并在中试生产中应用。获得１株阻断型突变株７－２５，其代谢产物经光谱学分析鉴定为ＪＩ－２０Ａ，并对其代谢途径进行了讨论。
Sisomicin high producer NS8-30 was isolated by conventional mutation breeding. As a result.the production of strain NS8-30 was increased by more than 17-fold,and was investigated in 5-ton fermentor.The blocked mutant 7-25 was obtained. By separating and purifying with IRC-50, antibiotic 7-25 was obtained as major component.It indicates that antibiotic 7-25 is identical with JI-20A by spectroscopy analysis.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1996年第1期5-8,共4页
Chinese Journal of Antibiotics
关键词
西索米星
突变育种
小单孢菌
制药
Sisomicin,Mutation breeding,Blocked-mutant, JI-20A
