摘要
以底物类似物抗性作为选择性标记来筛选高产海因酶的突变菌种。假单胞菌(Pscudomonas sp. )J43通过紫外光、亚硝基胍、亚硝基胍加5-氟尿嘧啶三次诱变处理后,测定了161株有5-氟尿嘧啶抗性的突变株的产海因酶活力,得到的突变株M39产海因酶活力比出发株提高了13.7倍。此法对选育其他酶或其催化产物的高产菌种可能也有意义。
Hydantoinase overproduction strains were bred by use of substrate analogue resistant mutation. Pseudomonas sp. J43 was treated by UV, NTG and NTG plus 5-FU sequencely. Mutant M39 which produced hydantoinase 13.7 folds higher than that of original strain was obtained from 161 strains of mutants with 5-FU resistance by determination of hydantoinase activity. The method should be applied to screen overproduction strains of other enzymes or their products.
出处
《微生物学报》
CAS
CSCD
北大核心
1995年第5期342-345,共4页
Acta Microbiologica Sinica
基金
"八.五"攻关课题部分内容
