摘要
目的:用大孔吸附树脂制备丹参总酚酸,测定树脂纯化后总酚酸的含量,并用HPLC图谱进行定性分析。方法:用树脂制备丹参总酚酸,用紫外分光光度法测定总酚酸的含量。以原儿茶醛为对照品,检测波长281nm;用HPLC法定性分析总酚酸的成分图谱,并对树脂纯化前后的图谱进行比较。采用Zorbax ODS柱(4.6 mm×250mm,5μm),以甲醇-1%冰醋酸水溶液梯度洗脱,检测波长281 nm。结果:树脂纯化后总酚酸的含量为53.8%;HPLC图谱定性分析表明采用此法能较好获得丹参中水溶性有效部位总酚酸。结论:采用含量测定和HPLC谱相结合的方法能更好地控制大孔树脂制备丹参酚酸的质量,有利于指导工艺研究。
Objective： To purify salvianolic acids by macroreticular resin, then mensurate the contents of salvianolic acids and analyse the chromatogram with HPLC. Method： Make salvianolic acids with macroreticular resin;mensurate the content of Salvianolic acids with UV spestrophotometry： the control compound is protocaechuic aldehyde, and the wavelength is 281 nm. Analysis the chromatogram with HPLC, and compare the chromatogram in different technics： zorbax ODS column（4.6mm×250mm, 5μm）, mobilephase： 1% aceticacid-water and methanol in different proportions, the wavelength is 281 nm. Result： The contents of salvianolic acids is 53.8% ;HPLC chromatogram indicate that the method is reasonable to make salvianolic acids. Conclusion： Determination of contents and HPLC chromatogram can control the quality of Salvianolic acids more accurately.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第17期1331-1334,共4页
China Journal of Chinese Materia Medica
