摘要
目的：研究Ｐ物质（ＳＰ）和ＳＰ受体在豚鼠耳蜗的分布．方法：采用兔抗ＳＰ血清、兔抗ＳＰ受体血清和免疫组织化学ＡＢＣ法及葡萄糖氧化酶－ＤＡＢ－镍（ＧＤＮ）染色技术．结果：在Ｃｏｒｔｉ器各圈均可见ＳＰ样免疫反应阳性的纤维和终末，以顶回及底回分布密集．阳性纤维从骨螺旋板向内外毛细胞区呈放射状分布，纤维呈串珠状，含明显的膨体．阳性的终末位于内、外毛细胞的底部．耳蜗各回均可见ＳＰ受体样阳性反应，其分布状态与ＳＰ阳性终末的分布状态相似，表现为内、外毛细胞底部的圆形、椭圆形结构．
Objective: To investigate the distribution of substance P (SP) and SP receptor (SPR) in the cochlea of guinea pigs. Methods: Rabbit serum against SP and SPR immunohistochemical ABC method and glucose oxidase DAB nickle technique were used in this study. Results: The results showed that SP IR positive fibers and terminals could be seen in all the four turns of the cochlea, densely populated in the apical and basal turns. The positive fibers emerged like a string of beads and were radially distributed from osseous spiral laminal to the inner and outer hair cell region. The positive terminals were located in the inner and outer hair cell bases. The SPR like positive products could also be seen in all the cochlear spirals. The distribution pattern of the SPR in the cochlea was similar to that of SP positive endings, either round or ovoid in shape. Conclusion: SP may play an important role in the primary auditory afferent processes.
出处
《第四军医大学学报》
1996年第1期1-4,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目
关键词
耳蜗
神经递质
P物质
受体
免疫组织化学
豚鼠
cochlea substance P receptor, substance P immunohistochemistry guinea pig
