摘要
90只清洁级昆明系小鼠,随机分为5组,预饲基础日粮一周后,各组分别灌胃:生理盐水(对照组,CK)、伴大豆球蛋白液(Ⅰ)、伴大豆球蛋白胃蛋白酶水解液(Ⅱ)、伴大豆球蛋白胃蛋白酶水解液提纯B组分(Ⅲ)、伴大豆球蛋白盐酸水解液(Ⅳ),除对照组外,各处理组灌胃液体总含氮量相等,实验期21d。结果显示:与对照组相比,第3周Ⅱ组和Ⅲ组每只小鼠日均采食量较对照组显著下降,分别降低8.75%(P<0.05)和9.28%(P<0.05);Ⅲ组小鼠胸腺相对质量极显著下降,降低了10.40%(P<0.01),肠黏膜sIgA水平提高45.49%(P<0.01),大肠食糜中6磷酸果糖酮糖酶(F6PPK)活性极显著提高(P<0.01),pH值下降了1.80%(P<0.05),肠球菌数显著下降(P<0.05)。实验结果表明水解肽作为腔内信号分子可能与肠道内相关因素有着多层次调控关系,共同促进宿主的健康。
Ninety male KM mice were fed basal diet during the first week and then randomly assigned five treatments. Each treatment was administered intragastrically for 21d with physiological saline （control group, CK）, conglycinin （ Ⅰ ）, pepsin-treated conglycinin （ Ⅱ ） and fraction B which isolated from pepsin-treated conglycinin （ Ⅲ ） and HCl-full hydrolysis of conglycinin （ Ⅳ ） respectively. Level of every mouse is 0. 5 mL with equal amount of nitrogen （0. 3 mg · mL^-1 ）. The results showed that, compared with control group, daily average feed intake of treatment Ⅱ and Ⅲ was decreased by 8. 75% （P 〈 0. 05 ） and 9. 28% （P 〈 0. 05） during the third week. The thymus relative weight of treatment Ⅲ was significantly decreased by 10. 40% （P 〈0. 01 ） and intestinal mucosal slgA levels increased by 45.49% （ P 〈 0. 05 ）. Enzyme activity of large intestinal contents fructose-6-phosphate phosphoketolase （F6PPK） was higher than control group （P 〈 0. 01 ）. pH value of large intestine contents was decreased by 1.80% （ P 〈0. 05） and total number of enterococcus also significantly decreased （ P 〈0. 05）. This study indicated that conglycinin peptides could improve the immunity of mice and may be beneficial in improving gastrointestinal health.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2005年第3期71-74,共4页
Journal of Nanjing Agricultural University
基金
国家自然科学基金资助项目(30070565)
