摘要
采用乙醇－氯仿处理、加热、丙酮沉淀和ＤＥＡＥ－ＳｅｐｈａｄｅｘＡ－５０柱层析的方法，从犬血红细胞中提纯了铜锌超氧化物歧化酶（Ｃｕ·Ｚｎ－ＳＯＤ），并对其部分性质进行了研究。比活力为４１５０Ｕ／ｍｇ，不连续聚丙烯酰胺凝胶电泳呈现１条区带，ＳＤＳ－聚丙烯酰胺凝胶电泳测得该酶的亚基分子量为１６３００，紫外最大吸收峰位于２５９ｎｍ，氨基酸组成中不含有色氨酸。
A copper and zinc-containing superoxide dismutase(Cu· Zn-SOD)waspurified from canine erythrocytes according to McCord and Fridovich’s method with alittle modification. The purified enzyme is homogeneous protein. Its specific activity is4 150 U/mg. The molecular weight of its subunit determined by SDS-PAGE is 16 300.It exhibits absorption maximum at 259 nm and contains no tryptophan.
出处
《中国兽医学报》
CAS
CSCD
1996年第2期170-172,共3页
Chinese Journal of Veterinary Science
关键词
铜
锌
超氧化物歧化酶
犬
红细胞
纯化
copper-zinc superoxide dismutase
canine
erythrocytes
purification
