摘要
超氧化物歧化酶（ＳＯＤ）是一种消除体内氧自由基损伤的重要因子，可分为ＣｕＺｎ－ＳＯＤ、Ｍｎ－ＳＯＤ和Ｆｅ－ＳＯＤ．作者在小白菜叶中曾发现５种ＳＯＤ，已鉴别其中一种为Ｍｎ－ＳＯＤ，来源于线粒体［１］；其余４种为ＣｕＺｎ－ＳＯＤ，其中２种分别来源于胞质［...
Three Cu·Zn SODs from the cytosol of Allium schoenoprasum L.have been purified to homogeneity by ammonium sulfate precipitation,gel filtration on Sephadex G 200 and chromatography on DEAE Sephacel.SOD Ⅰ,SOD Ⅱ and SOD Ⅲ all gave ultraviolet absorption maximum around 268 nm and visible absorption maximum around 670 nm.Of the three SODs,the molecular weights were 31 600,31 200 and 33 100;the subunit molecular weights were 16 000,16 200 and 16 400;the isoelectric points were 6.10,6.10 and 5.90;the N terminal amino acids were all alanine.Their heat stability was fine.SOD Ⅰ and SOD Ⅱ were very similar.There were some differences between them and SOD Ⅲ on isoelectric points,sensitivity to KCN or heat,the quantity in cytosol and electrophoretograms of nondenaturing polyacrylamide gel electrophoresis.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第6期796-799,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
湖北省科委重点科技项目
关键词
小葱
Cu.Zn-SOD
纯化
性质
Cu·Zn SOD, Allium schoenoprasum L.,Cytosol,Purification,Property
