摘要
目的:检测急性冠脉综合征(ACS)患者外周血单核细胞中环氧化酶2(COX-2)mRNA的表达水平并探讨其意义。方法:选择典型急性冠脉综合征患者42例,分离和培养单核细胞,并采用不同浓度的选择性COX-2抑制药(塞来昔布)体外干预。采用RT-PCR法半定量COX-2mRNA的表达,夹心酶联免疫吸附法(ELISA)测定培养上清液中IL-6和基质金属蛋白酶9(MMP-9)浓度。结果:ACS患者外周血单核细胞中COX-2mRNA表达(0.61±0.17)较健康对照组(0.11±0.09)明显增强(P<0.05);ACS患者外周血单核细胞培养上清液中IL-6浓度[(97.24±11.21)ng/L]较健康对照组[(22.15±6.30)ng/L]显著升高(P<0.05),MMP-9浓度[(41.20±8.41)μg/L]较健康对照组[(11.76±4.23)μg/L]显著升高(P<0.05);ACS患者外周血单核细胞经塞来昔布干预后,塞来昔布以浓度依赖性方式降低培养上清液中IL-6和MMP-9浓度分别达48%和50%(均P<0.05)。结论:COX-2可能在急性冠脉综合征的炎症反应中起重要作用。
Objective To determine the expression of cyclooxygenase-2 （COX-2） mRNA in peripheral blood monocytes in patients with acute coronary syndrome （ACS） , and to explore the effect of the expression of COX-2 mRNA in ACS. Methods The expressions of COX-2 mRNA in peripheral blood monocytes from 18 normal subjects and 42 ACS patients were analyzed by reverse transcription polymerase chain reaction （RT-PCR） , and the monocytes from patients were incubated with celecoxib in vitro. The concentrations of interleukin-6 （IL-6） and matrix metalloproteinase-9 （MMP-9） in supernates of mono- cytes were measured by enzyme-linked immunosorbent assays （EL1SA）. Results The expression of COX-2 mRNA and tile secrections of IL-6 and MMP-9 in peripheral blood monocytes in ACS patients significantly increased compared with those from normal controls [0.61±0.17 vs 0.11±0.09; （97.24±11.21）ng/L vs （22.15±6.30）ng/L; （41.20±8.41） g/L vs （11.76±4.23） g/L; all P〈0.05, respectively] . Celeeoxib reduced IL-6 and MMP-9 secretion level of monoeytes from ACS patients up to 48% and 50% respectively （ all P 〈0.05）, in a concentration-dependent manner. Conclusion COX-2 in peripheral blood monocvtes may play an important role in the acute coronary syndrome.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2005年第4期403-406,共4页
Journal of Central South University ：Medical Science
基金
长沙市医药卫生科技计划项目(2002-0211)
