摘要
目的:研究电离辐射对人成纤维细胞中透明质酸酶(HAase)表达的影响,并探讨其在放射性臂丛神经损伤致病机制中的作用。方法:0.5、1.0、2.0、4.0和6.0Gy X射线分别照射人成纤维细胞24和48h,同时设立假照组(0Gy X射线),透射电镜观察其超微结构的改变,并采用ELISA法和Real-time PCR法检测HAase蛋白及mRNA表达的变化。结果:照射48h后,2.0Gy剂量组细胞核内染色质开始聚集;4.0Gy剂量组细胞核染色体出现碎裂,染色体开始出现空泡化;6.0Gy剂量组细胞核染色质不再明显,呈现早期细胞凋亡改变。在照射24和48h后,HAase蛋白的表达随着照射剂量的增加呈下降趋势,且与假照射组比较差异有统计学意义(P<0.05);照射24h后,0.5和4.0Gy剂量组HAase mRNA表达均高于假照射组(P<0.05),1.0、2.0和6.0Gy剂量组HAase mRNA表达降低,与假照射组比较差异有统计学意义(P<0.05);照射48h后,2.0、4.0和6.0Gy剂量组HAase mRNA表达高于假照射组(P<0.05),0.5和1.0Gy剂量组HAasemRNA表达则明显低于假照射组(P<0.05)。结论:电离辐射剂量的增高可使人成纤维细胞HAase的表达降低,对放射性臂丛神经损伤的发生有一定的影响。
Objective To study the effect of ionizing radiation on the expression of hyaluronidase(HAase) in human fibroblasts and to discuss the role of HAase in the pathogenic mechanism of radiation-induced brachial plexus injury.Methods The fibroblasts were irradiated for 24 and 48 h respectively by X-ray with doses of 0.5,1.0,2.0,4.0 and 6.0 Gy,and sham irradiation group(0 Gy X-ray) was set up.The ultrastructural changes of fibroblasts were observed by transmission electron microscope,the expression changes of protein and mRNA of HAase were detected by ELISA and real-time PCR method.Results After irradiated for 48 h,the nuclear chromatin began to get together in 2.0 Gy group;the nucleus and chromosome fractured and the chromosome appeared empty bubble change in 4.0 Gy group;the chromatin was no longer apparent and the apoptosis change presented early in 6.0 Gy group.After irradiated for 24 and 48 h,with the increasing of the radiation dose,the expression of HAase protein declined,there was significant difference compared with sham irradiation group(P<0.05).The expressions of HAase mRNA in 0.5 and 4.0 Gy groups were significantly higher than that in sham irradiation group(P<0.05),but the expressions of HAase mRNA in 1.0,2.0 and 6.0 Gy groups were significantly lower(P<0.05) at 24 h.The expression of HAase mRNA in 2.0 Gy,4.0 Gy and 6.0 Gy groups were significantly higher than that in sham irradiation group at 24 h(P<0.05),the expressions of HAase mRNA in 0.5 Gy and 1.0 Gy groups were significantly lower at 48 h(P<0.05).Conclusion The increase of the radiation dose could reduce the expression of HAase in human fibroblasts,and influence the occurrence of radiation-induced brachial plexus injury.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2011年第6期989-993,共5页
Journal of Jilin University：Medicine Edition
基金
国家自然科学基金资助课题(30973057)
吉林大学基本科研业务费项目资助课题(200903117)
吉林省科学技术教育厅"十二五"科学技术研究项目资助课题[吉教科合字(2011)第154号]
吉林省人才开发基金资助课题(802090000430)
吉林省科技厅科技发展技术项目资助课题(200705269)
关键词
辐射
电离
成纤维细胞
透明质酸酶
透明质酸
放射性臂丛神经损伤
radiation,ionizing
fibroblasts
hyaluronidase
hyaluronic acid
radiation-induced brachial plexus injury
