摘要
目的 探讨外源性透明质酸 (HA)抑制瘢痕形成的作用机理及其浓度依赖性关系。方法 健康成年大鼠 4 5只 ,复制背部创伤模型 ,随机分成 2 %HA治疗组 (A组 )、1%HA治疗组 (B组 )、磷酸盐缓冲液 (PBS)对照组 (C组 ) ,观察大体形态和组织学改变 ,平均愈合时间和残留面积的大小 ,测定愈合部位组织蛋白、羟脯氨酸 (HPr)、Ⅲ型前胶原 (PCⅢ )的含量。结果 ① 3组的平均愈合时间为 ( 15 2± 2 0 ) ,( 14 2± 1 6)和 ( 13 4± 1 4 )d ,A、B两组与C组比较差异有显著性意义 (P <0 0 5 )。A、B两组的残留面积与C组比较差异有显著性意义 ,3组间比较差异也有显著性意义 (P <0 0 5 )。②组织学上 ,A、B两组成纤维细胞增生明显 ,胶原纤维直径较为纤细、排列整齐。C组则显示出明显的炎性细胞浸润 ,新生血管数目增多。③HPr和PCⅢ含量的测定 :A、B两组HPr含量的均值低于C组 (P <0 0 1) ,而PCⅢ的含量却高于C组 (P <0 0 1)。结论 ①HA通过对细胞的调控作用 ,可有效地抑制胶原的合成 ,提高Ⅲ型胶原含量 ,使得创面愈合过程中瘢痕形成减少。②HA在创面愈合中抑制瘢痕形成的生物学作用与其浓度成正比关系。
Objective\ To evaluate the effects and possible mechanisms of hyaluronic acid (HA) to inhibit scar formation as well as the dose dependent relationship.Methods\ Forty five health adult SD rats in a dorsal trauma model were randomly divided into the 2% HA treated group( group A),1% HA treated group(group B),and the PBS treated group as a control group(group C).The process of healing was observed,the remained wound areas were measured,and a mean wound healing time was calculated.Furthermore, the dynamic analysis was performed on local tissue protein,HPr and PCⅢ contents.Results\ ①The remained wound areas of the control group were much smaller than that of group A and B ,and the mean value of wound healing time was 15 2±2 0,14 2±1 6 and 13 4±1 4 days respectively.②Histologically,when compared qualitatively with PBS controls,HA treated wounds exhibited a less inflammatory response and angiogenesis. The fibroblast proliferated significantly,and the collagen fibril was slender and arrayed in good order,③The contents of tissue HPr were statistically lower in HA treated groups,while the contents of PCⅢ were statistically higher compared to the control group.Conclusions\ The study indicated that ①The exogenous hyaluronan acid may effectively inhibit collagen synthesis and increase tissue PCⅢ content.As a result,the scar formation was decreased.②Hyaluronal efficacy presented a dose dependent manner.\;
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2000年第1期30-33,共4页
Chinese Journal of Plastic Surgery
