摘要
【目的】通过喉狭窄瘢痕组织的病理学研究,了解炎症细胞在狭窄瘢痕组织表达及意义。【方法】垂直喉部分切除术后2～3月出现喉狭窄10例和未狭窄16例喉瘢痕肉芽组织为实验组及10例正常声带组织为对照组,分别进行苏木精-伊红及免疫组化染色,观察组织切片中中性粒细胞、巨噬细胞、T淋巴细胞表达,并在全自动图像分析系统下进行细胞计数。【结果】喉狭窄瘢痕组织与非狭窄喉瘢痕组织、正常声带比较,固有层炎症细胞大量聚集,主要以巨噬细胞和T淋巴细胞为主。比较非狭窄喉瘢痕组织及正常声带组织,喉狭窄瘢痕组织的中性粒细胞、巨噬细胞、T淋巴细胞数量均明显增加,差异有统计学意义(P<0.01)。【结论】喉狭窄瘢痕肉芽组织中存在明显炎症反应,持续炎症反应可能是导致喉狭窄瘢痕肉芽增生重要因素之一。
[Objective] To explore the significance of the expression of inflammation cell in the laryngeal stricture scar by the study of the pathological features of laryngeal stenosis scar. [Methods] The laryngeal tissues were 10 human surgical specimens of laryngeal stenosis scar and 16 human surgical specimens of laryngeal scar which no-stenosis after vertical partial laryngectomy; 10 human normal vocal folds were control. Expression of neutrocytes, macrophage, and T lymphocyte were observed by Van Gieson and immunohistochemistry staining respectively in laryngeal tissue sections. The amount of inflammatory cells were analyzed with KONTRON IBAS 2.5 automatic image analysis system. [Results] The soakage density of neutrocytes, macrophage, and T lymphocyte were high in inhesion layer of laryngeal stenosis scar. The count of neutrocytes, macrophage, and T lymphocyte in laryngeal stenosis group were significantly higher than those in the no-stenosis scar group and normal vocal folds group （P 〈 0.01）. [Conclusion] There were obvious inflammation reaction in the laryngeal stenosis scar. Persistence inflammation reaction may play an important role in the hyperplasia of laryngeal stenosis scar.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2006年第5期545-548,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省自然科学基金资助项目(31733)
关键词
垂直喉部分切除术
喉狭窄/病理学
炎症细胞
vertical partial laryngectomy
laryngeal scar/pathohistology
inflammatory cells
