摘要
采用荧光分光光度法测定人尿液中诺氟沙星的排出速率，尿样不经处理，以０．０５ｍｏ１／Ｌ盐酸稀释后用扣除空白尿样的方法进行测定。当诺氟沙星的浓度为０．０５～０．５μｇ／ｍｌ范围内，其荧光强度与浓度线性关系良好。该法的最低检出量为０．０１μｇ／ｍ１，平均回收率在９８％以上。本文采用此法测定了３名健康受试者口服诺氟沙星胶囊后的尿药排出速率，并绘制出排出速率曲线。
A fluorescence spetrophotometry was developed for the determination of the excretion rate of norfloxacin in urine,Urine samples were determined by 0.05 mo1/LHCI dilution andafter deduction blank of urine sample。The detection limits of norfloxacin was 0.01 βg/ml,and thecalibration curve was linear(r=0.9998)in the concentration range of 0.05～0.5βg/m1.Themean recovery of norfloxacin was over 98%。 The excretion rate of norfloxacin in urine for threeheaIth volunteers following per os of norfloxacin capsuls was determined by this method.Excretion rate curves of norfloxacin were drawn。
出处
《中国药学杂志》
CAS
CSCD
北大核心
1995年第7期428-430,共3页
Chinese Pharmaceutical Journal
关键词
诺氟沙星
尿液
排出速率
荧光分光光度法
norfloxacin,urine,excretion rate,fluorescence spetrophotometry
