摘要
本文以ＲＨ株细胞培养上清（ＳＲＣ）替代ＰＬＡ２加入培养系统，对三株成囊株４次培养及与含ＰＬＡ２对照培养的观察表明，含ＳＲＣ的培养系统较常规培养检出时间提前，阳性率提高，在本文条件下与含ＰＬＡ２４０Ｕ者效果相当，提示ＳＲＣ可作为ｐＬＡ２的廉价实用的替代物用于弓形虫病的病原检测。
The authors reported previously that the exogenous phospholipase A2(PLA2)intoxoplasma tissue culture could remarkably enhance the invasion of tachyzoites to host cells. Inthe further studies we observed that supernatant of RH strain culture(SRC)can improve the cellculture of cytogenic strains especially for that with smaller quantity of inoculum. In the inoculumof l00 tachyzoites the positive results were obtained earlier than that of control. This resuIt indi-cated that SRC can replace PLA2 in tissue culture for pathogenic diagnosis of toxoplasmosis.
出处
《中国寄生虫病防治杂志》
CSCD
1995年第2期111-113,共3页
Chinese Journal of Parasitic Disease Control
关键词
弓形虫
细胞培养
RH株培养
Toxoplasma gondii cell culture RH strain culture
