摘要
用HeLa细胞系研究了自国内分离的3株弓形虫的生长动态,并与RH株进行了比较。RH株弓形虫速殖子与HeLa细胞单层接触,只需2min,虫体便可侵入;而CN株需5min,ZS_2和PP株需10min。虫体侵入HeLa细胞至开始增殖,约经6h的迟滞期。通过计算各株不同孵育期纳虫泡内虫体数以及直线回归方程处理,发现RH株增殖一代的时间是5.2h,CN株是5.98h,ZS_2株是6.78h,PP株是7.69h。3株弓形虫中,以CN株对HeLa细胞的侵入力和增殖力与RH株最接近。
Growth dynamics of PP, CN and ZS2 strains of Toxoplasma gondii isolated in China was studied and compared with that of RH strain. HeLa cells were used in this work. It took only 2 min for the organisms of RH strain to infect the HeLa cells when con-tacting with the cells. By contrast, the CN strain requires 5 min, the ZS2 and PP strains, 10 min.Tcxoplasma began its multiplication after a lag time of about 6 h in the HeLa cells. The mean generation time of the 4 strains was assessed by calculating the number of the parasites in the parasitophorous vacuoles at different incubation times and by the linear regression equation. The results showed that the mean generation time was 5.2 h for RH strain, 5.98 h for CN strain, 6.78 h for ZS2 strain and 7.69 h for PP strain.Among the 3 strains of Toxoplasma gondii isolated in China, CN strain was similar to RH strain in their infectivity and proliferation.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1989年第1期8-11,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
中国科学院科学基金资助
