摘要
以芥菜型油菜子叶和下胚轴来源的愈伤组织为材料，进行悬浮细胞振荡培养，通过诱导愈伤组织及分化试验表明：用乳白色且结构紧密的愈伤组织进行悬浮振荡培养能获得较正常的单细胞；在悬浮振荡培养的第６～７天内及时过滤并将单细胞转入浅层液体分正培养，可高频率产生意伤组织；愈伤组织的质量对芽的分化有重要影响，不同基因型来源的愈伤组织其植株再生频率有很大差异。通过试验在云南芥菜型油菜中发现了一批易再生材料并建立了体细胞无性系。
Cell suspension of calli from cotyledon and hypotyl of Brassica juncea were suggested:the good isolated single cells which can high-frequently produce calli could be obtained from calli with white colour and close structure;the quality of calli effected importantly on the regeneration of shoots;the regeneration frequency was different for calli from different genotypes.Some materia is wilh high ability of regeneration were observed in Brassica juncea of Yunnan and a somaclone was established.
出处
《西南农业学报》
CSCD
1995年第3期27-30,共4页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金
云南省应用基础研究基金
