摘要
利用ＣＤ谱对皖南尖吻蝮蛇蛇毒内抗凝血因子（ＡＣＦ）的二级结构，即α－螺旋、β－折叠和无规则卷曲进行了测定，利用ＣｈｅｎａｎｄＹａｎｇ的方法计算出了它们在ＡＣＦ分子内的百分比．溶液的ｐＨ值对ＡＣＦ的二级结构影响不大，但当ｐＨ位于５和６时，二级结构稍稍出现了异常．这可能是由于氢离子电离引起的电荷变化带来的效应．ＡＣＦ的脱钙破坏了分子内的配位结构，而使α－螺旋的百分比大大降低．尽管三价镧系离子能取代ＡＣＦ中的钙离子，但是没有给ＡＣＦ的二级结构带来较大的影响．
he secondary structure of Anticoagulation Factor (ACF) in the venom of agkistrodon acutus, i. e. α-helix, β-sheet and random coil, have been studied by CD spectroscopy, and their percentages have been also obtained from the calculation based on Chen and Yang Equation.The effect of pH on the secondary structure of ACF is small, while an bit abnormal changes on pH = 5 and 6, observed in H2O percentages, is possibly caused by the changes of electric charges during the ionization of protons on the amino acid residures. The decalification of ACF results in a great decrease for the α-helix, due to the destruction of coordination between Ca(2+)and the ligand groups in ACF molecule. The lanthanide ions, for example, Tb(3+), Nd(3+) and Eu(3+), do not effact the secondary structure of ACF, even they can subsititute for Ca(2+) ion in ACF.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
1995年第1期78-82,共5页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金
关键词
蛇毒
抗凝血因子
二级结构
CD谱
金属离子
snake venom
anticoagulation factor(ACF)
secondary structure
CD spectroscopy
