摘要
由紫外光谱法测定蛋白质浓度,用ICP测定Ca含量,从而确定一个ACF分子中含一个钙离子.ACF中分子中除一个高亲和性钙结合位点外,至少还有一个低亲和性钙结合位点,只有当过量钙离子存在时,才可能在低亲和性钙结合位点上进一步结合钙离子.Tb^(3+)具有比Ca^(2+)离子更强的键合ACF能力,能定量结合在ACF中的两个位点上,且能全部取代ACF中Ca^(2+).文中提及的金属离子对ACF的抗凝血活性没有显著的影响.
The corrective coefficient of the equation on determination of the concentration of ACF by UV spectral (280nm-260nm) has been introduced. Our studies show that each ACF molecule contains one of Ca2+ ion, and has two Ca2+-binding sites at least (stronger one and weaker others). Ca2+ ion can bind to the weaker site in ACF only in the condition of existence of excessive Ca2+ ion. Tb3+ ion is coordinated with ACF more strongly than Ca2+ ion, while Tb3+ ion can bind to ACF at both two sites quantitatively and replace of Ca2+ ion in ACF completely. All the metal ions mentioned in this paper do not give intensive effect on the anticoagulation activity of ACF.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
1993年第1期71-76,共6页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金
关键词
抗凝血因子
钙
铽
荧光
尖吻蝮蛇
anticoagulation factor, calcium, terbium, fluorescence
