摘要
采用高效离子交换色谱法对经初步分离的皖南尖吻蝮蛇毒活性成分进行进一步的分离纯化。采用线性梯度洗脱，得到蝮蛇毒活性组分纯品，并用峰面积归一化法计算其含量为４５．４１％左右，纯度在９０％以上。
The active component from Agkistrodon acutus snake venom was further purified by high performance ion exchange liquid chromatography over a Protein PAKTM DEAE 8HR column(100mm i.d.×100mm). The elution was operated with a linear gradient from 10% to 100% NaCl 002mol/L Tris HCl buffer(pH 80).The quantitative results were calculated as 4541% based on peak area nomalization and the purity of the active component was 90% determined by electrophoresis.
出处
《色谱》
CAS
CSCD
北大核心
1998年第3期261-262,共2页
Chinese Journal of Chromatography
关键词
皖南
尖吻蝮蛇毒
活性组分
蝮蛇毒
蛇毒
分离
high performance ion exchange liquid chromatography, Agkistrodon acutus snake venom, active component
