摘要
通过研究神经节苷脂ＧＭ＿３对国人单核样白血病细胞系Ｊ６－２细胞蛋白质磷酸化的影响，在［γ－￣３２Ｐ］ＡＴＰ，ＧＭ＿３，ＡＴＰ，Ｍｇ￣（２＋）与Ｊ６－２细胞液及颗粒两部分共同反应，１Ｏｍｉｎ（３０℃）体系中，观察到ＧＭ＿３对两部分蛋白质磷酸化的调节作用。ＧＭ＿３（１００μｍｏｌ／Ｌ）促进颗粒部分分子量为１８００００，８７０００，７８０００，６７０００，４３０００及３１０００的蛋白质磷酸化，促进胞液部分分子量为８７０００及５６０００的蛋白质磷酸化，而且能抑制７００００及４３０００蛋白质磷酸化。由于ＧＭ＿３已被前人证实能对Ｊ６－２细胞起分化作用，其作用时间长达４－６ｄ，很可能ＧＭ＿３对蛋白质磷酸化作用的调节是ＧＭ＿３促分化作用的早期信号。
The effect of ganglioside GM_3 onthe endogenous protein phosphorylation of hu-man monocytoid leukemia cell line J6-2 cellswas studied.Addition of 100 μmol/L GM_3 tothe particulate fraction of J6-2 cells enhancedthe endogenous phosphorylation of severalproteins whose relative molecular mass were180,87,78,67,43 and 31kD while additionof 100 μmol/L GM_3 to the cytosol fraction of16-2 cells stimulated the phosphorylation ofseveral proteins whose relative molecular masswere 87 and 56kD,but could inhibit the phos-phorylation of 70 and 43kD proteins。BecauseGM_3 has been reported to be able to induce J6-2 cells to differentiate along the monocyte/macrophage route by long term incubationwith GM_3(up to six days),so the authorspostulated that protein phosphorylation in-duced by short term GM_3 treatment might bethe early signal of differentiation of J6-2 cells.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1995年第1期56-59,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金
关键词
神经节苷脂
白血病
细胞
蛋白
磷酸化
ganglioside GM_3,protein phos-phorylation,leukemic cells
