摘要
从正常兔、猪和狗的肝脏及狗红细胞中分离纯化了总神经节苷脂,测定了脂结合唾液酸,进行了高效薄层层析,比较了上述四种组织中GM_3的含量。结果表明狗红细胞中的GM_3的含量较另三种的高,狗肝和兔肝次之,猪肝含量甚微。从狗红细胞中提取和纯化了GM_3,其得量为每毫升压积红细胞351.0μg,纯度为92.2％。
Total gangliosides were isolated from the liver of healthy rabbit, pig, canine, and canine erythrocytes. After high performance thin layer chromategraphy of the total gangliosides, the chromatograms were scanned by TLC scanner, the percentage contents were obtained. On the basis of lipid-bound sialic acid contents, the authors found that canine erythrocyte had the highest GM_3 content. GM_3 isolated from compacted canine erythrocytes with a yield of 351.0μg per ml and the purity was 92.2％.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1990年第3期206-209,共4页
Progress In Biochemistry and Biophysics
