摘要
通过苔酚蓝染色细胞发现，外源性GM3（10μg/ml）能明显抑制人肝癌细胞株SMMC-7721细胞生长，在GM3处理3d时，出现明显差异．通过NorthernBlot分析发现，外源性GM3可明显影响人肝癌细胞株SMMC-7721细胞中c-fos、c-jun、c-myc和N-ras这四种癌基因的mRAN表达．未经GM3处理的细胞中没有检测到c-fosmRNA，但c-jun微量表达，并有c-myc和N-rasmRNA的高水平表达而GM3可在短时间内快速大量地诱导c-fos、c-junmRNA的生成．GM3处理的细胞，c-myc和N-rasmRNA的表达均明显减少．GM3处理45min时，c-myc基因表达只为对照组的39．55％；GM3处理24h时，N-ras基因表达为对照组的30.48％．以上结果提示：GM3抑制SMMC-7721细胞生长很可能是通过改变癌基因表达来实现的．
By counting living cells with Trypan Blue staining, it was found that 10 μg/ml of GM3 could inhibit the proliferation of SMMC-7721 human hepatocarcinoma cells. It was found by Northern blot anslysis that c-fos.c-jun.c-myc and N-ras oncogenes mRNA expression in SMMC7721 cells could be affected by exogenous GM3. The results showed that c-fos mRNA could not be detected and c-jun expressed very little in the untreated cells,whereas their transcription could be induced transiently by GM3 in very short time. GM3 could inhibited c-myc mRNA expression with a decrease of 60. 45 % as early as 45 minutes after treatment. The expression of N-ras oncogene,one of the transforming genes of human heptocacinona cells, was promptly declined (69.52% ) after exposure to GM3 for 24 hours. The results suggested that effects of exogenous GM3 on SMMC-7721 cells growth may be finally carried out by the way of changing expression of some oncogenes.
基金
国家自然科学基金!39040001
中国科学院上海生物比学研究所分子生物学重点实验室客座课题
关键词
神经节苷脂GM3
肝癌
细胞生长
Ganglioside GM3, The growth of human heptocarcinoma cell, c-fos, c-jun, c-myc and N-ras oncogenes
