摘要
本文报道ＡＢＴ附加于康乃馨生根培养基和直接用于试管苗瓶外生根的效果．结果显示，附加ＡＢＴ培养基培养的康乃馨试管苗与附加ＮＡＡ，ＩＢＡ和对照培养基培养的试管苗相比，其生根率及移栽存活率均有显著或极显著的提高，ＡＢＴ诱导试管苗生根的适用范围与ＩＢＡ一致，适宜浓度为０．２５～１ｍｇ／Ｌ，抑制浓度为４ｍｇ／Ｌ以上。诱导康乃馨试管苗瓶外生根，以１００ｍｇ／ＬＡＢＴ处理１ｈ效果最好，生根率达９０．７％，显著高于ＩＢＡ、ＮＡＡ处理的试管苗生根率，极显著高于对照试管苗生根率。
The effect of ABT on root initiation from tissue culture of carnation was studied by varying theABT concentrations in the rooting medium.It was found that the frequency of rooting and seedling survivalin the ABT trcjtment were increased significantly as campared with NAA,IBA and control treatmeat.Theconcentration of ABT and IBA on rooting initiation is simUar,about 0.25～1mg/L,but 4mg/L would inhibit root formation.On the other hand,when the shoot was treated in 100mg/L ABT solution for an hour,the rooting frequency was 90.7%,which was significant highs at 0. 5 level than IBA or NAA treatmentand the most significant higher at 0. 01 level than control treatment.
基金
上海市农科院青年基金
关键词
康乃馨
试管苗
生根
生根粉
ABT
Carnation
Tissue culture
Rooting
