摘要
从碱湖鱼内脏中分离筛选出1株碱性脂肪酶的高产菌株N4-2,初步鉴定为镰孢霉属(Fusarium)。摇瓶产酶实验表明,该菌株最适产酶培养基的组成为(g/L):橄榄油30,酵母膏5,(NH4)2SO43,MgSO40·75,CaCl20·2,K2HPO44。最适产酶温度30℃,最佳产酶pH为10·0,生长高峰出现在第43h,产酶高峰出现在第52h,碱性脂肪酶的活力高达605·15U/mL,此菌株生长周期短,产酶活力较高,在洗涤制剂、印染、制革等行业有良好的应用前景。
An alkaline lipase-producing strain was isolated from entrails of fish collected from alkaline lake and was identified as Fusarium. The optimal conditions for lipase production were determined as follows（g/L） ： olive oil 30, yeast extract 5, （NH4）2SO4 3, MgSO4 0.75, CaCl2 0.2, K2HPO4 4. The optimal lipase-producing temperature and initial pH were 30℃ and 10.0 respectively. When this strain was cultured for 52 hours in the optimal lipase-producing culture medium, the alkaline lipase activity reached maximal level of 605.15 U/mL. This strain has shorter growth cycle and higher lipase activity. The outlook of some potential applications in detergent, leather and other industries of this strain are promising.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2005年第7期31-34,共4页
Food and Fermentation Industries
