摘要
对一株宇佐美曲霉进行紫外光、微波和60 Co的逐级诱变育种 ,使酸性蛋白酶产量从 2 80 0U/mL提高到 72 0 0U/mL .突变株传代多次 ,产酶性能保持稳定 ;对突变株与出发株的发酵过程进行了比较研究 ,发现突变株最大产酶时间提前 10h以上 ,而培养基
The original strain Aspergillus usamii WX ZL10 was mutated by ultraviolet radiation, microwave radiation and γ ray. A mutant Aspergillus usamii 9169 was obtained. The yield of acid protease was improved from 2 800U/mL to 7 200U/mL. The mutant strain maintains perfect genetic stability after several generations of reproduction. The time profiles of both the mutant and its parental strain were plotted in which some of their physiological properties were compared. By contrast of its parent's, the optimal duration for obtaining maximal enzyme activity of the mutant was 10hrs decreased. The pH value of the medium had great impact on the biosyntheses and secretion of the acid protease.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2001年第5期493-496,共4页
Journal of Wuxi University of Light Industry
